Genetic linkage analysis of stable qtls in Gossypium hirsutum ril population revealed function of GhCesA4 in fiber development

Document Outline

• Genetic linkage analysis of stable QTLs in Gossypium hirsutum RILpopulation revealed function of GhCesA4 in fiber development
  • Introduction
  • Materials and methods
    • Plant materials and phenotype evaluation
    • DNA extraction and library construction and genotyping
    • HDGM construction and QTL mapping
    • Gene identification and annotation
    • RNA-Seq dataset collection
    • Identification of co-expression modules during fiber development
    • Enrichment analysis of protein–protein interaction (PPI) network and screen of candidate genes
    • Analysis of real-time quantitative polymerase chain reaction (qRT-PCR)
    • VIGS and CRISPR/Cas9 verification of GH_D07G2262
    • Paraffin cross-sectioning of natural mature fibers
  • Results and analysis
    • Phenotypic assessment of traits of fiber quality and yield
    • Construction of HDGM
    • QTLs and QTL clusters
    • Qtls common to those of previous studies
    • Construction and identification of the gene co-expression modules of stable QTLs and clusters in fiber development
    • Screening for candidate genes
    • Silencing verification of GH_D07G2262 function in cotton
  • Discussion
    • HDGM construction and QTL identification
    • QTL effect direction of each trait in QTL clusters and simultaneous improvement of the clustered traits
    • GhCesA4 may regulate cotton fiber development
  • Ethical statement
  • CRediT authorship contribution statement
  • Declaration of competing interest
  • Acknowledgements
  • Appendix A Supplementary material
  • References