Kolonkalar: Yog’da eruvchan vitaminlar uchun Acclaim PA, 3 µm, 3.0 × 150 mm for water-soluble vitamins Acclaim C18, 3 µm, 3.0 × 150 mm. Kolonka harorati

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Kolonkalar: Yog’da eruvchan vitaminlar uchun Acclaim PA, 3 µm, 3.0 × 150 mm for water-soluble vitamins Acclaim C18, 3 µm, 3.0 × 150 mm.
Kolonka harorati: 25 °C
Harakatchan faza: suvda eruvchan vitaminlar uchun A) 25 mM fosfat bufer (pH=3.6), B) CH₃CN-harakatchan faza A (7:3, v/v)
Yog’da eruvchan vitaminlar uchun, A) CH₃OH-CH₃CN (8:2, v/v), B) Metil tert-butil efir (MTBE).
Ikki analiz jarayoni ham gradientli usulda boradi (1-jadval).
Flow Rate: 0.5 mL/min
UV Detection: UV har bir vitaminlar uchun turli to’lqin uzunliklarida.
Ineksiya hajmi: 10 µL

1-rasm. 21 ta suvda va yog’da eruvchan vitaminlarning bir vaqtda ajralishidan hosil bo’lgan xromatogrammalar. Cho’qqilar: 1. Tiamin 2. Vitamin C 3. NC 4. Piridoksal gidroxlorid 5. Piridoksin gidroxlorid 6. Nikotinamid 7. Pantoten kislota 8. Foliy kislota 9. Siankobalamin 10. Riboflavin 11. Vitamin A 12. Lutein 13. Vitamin A atsetat 14. Vitamin D2 15. Vitamin D3 16. Vitamin E 17. Vitamin E atsetat 18. Vitamin K1 19. Likopen 20. Vitamin A palmitat 21. ß-Karoten.

Vitaminlarni ajratishda ishlatiladigan Acclaim kalonkasini tarkibi (A) 120 C18, (B) PA, va (C) PA2.
Metodikasi:

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